PROTHROMBIN

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Induction of prothrombin synthesis by prothrombin fragments.

The mechanisms by which blood levels of prothrombin (PT) are regulated in the vitamin K-sufficient state are unknown. We have studied PT synthesis by Reuber H-35 rat hepatoma cells exposed to vitamin K and [3H]leucine in serum-free cultures. Administration to the culture system of exogenous bovine PT and rat PT was characterized by increases in endogenous PT synthesis and secretion of 2- and 3-...

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Prothrombin Molise: a "new" congenital dysprothrombinemia, double heterozygosis with an abnormal prothrombin and "true" prothrombin deficiency.

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Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions.

Staphylocoagulase (SC) is a potent nonproteolytic prothrombin (ProT) activator and the prototype of a newly established zymogen activator and adhesion protein family. The staphylocoagulase fragment containing residues 1-325 (SC-(1-325)) represents a new type of nonproteolytic activator with a unique fold consisting of two three-helix bundle domains. The N-terminal, domain 1 of SC (D1, residues ...

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Human prothrombin activation.

Human prothrombin has been purified from American Red Cross Factor IX concentrates. Studies of the activation of the human prothrombin with the use of sodium dodecyl sulfate electrophoretic analysis of activation products indicated that human prothrombin activation is similar to bovine prothrombin activation. Molecular weight analysis of human prothrombin and intermediated by sodium dodecyl sul...

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Prothrombin Deficient Plasma

ASSAY PRINCIPLE: The method is a clotting assay where all the clotting factors are present (constant and in excess, brought by the deficient plasma), excepted for FII, which is brought by the diluted tested plasma, and clotting is triggered with calcium thromboplastin. FII is the limiting factor and clotting time is inversely proportional to the concentration of FII. There is an inverse linear ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1963

ISSN: 0021-9746

DOI: 10.1136/jcp.16.2.187-c